9/23/2023 0 Comments Bio rad gene pulser iiThus marine Chlorella strains have particular promise because they can grow on non-arable land and utilize saline water supplies. The use of marine Chlorella strain in industrial systems is attractive due to the ever-expanding demand for fresh water, which is estimated to increase ~ 30% by 2050. include both freshwater and marine strains. They are used as a food substitute for humans and are widely produced as health food in USA, Germany, China, Japan, and several other Asian countries. have been cultivated in huge quantities to meet the growing demand for alternative protein sources due to their short life span, robust environmental tolerance, and high content of proteins. The plant nature enables Chlorella as an excellent model organism to investigate the photosynthesis mechanisms and CO 2 assimilation (Calvin–Benson cycle) from 1940s. Up to now, only a few taxa are utilized for human consumption while Spirulina and Chlorella dominate the microalgal market. Although microalgae have been used for thousands of years, the exploitation of the biological diversity of microalgae and their application in human nutrition is constrained by regulations. Microalgae convert CO 2 into various bioresources for human domestic and industrial consumption. Microalga-based carbon neutralization holds great promise for net zero or negative emission production in commercial scales. The development of a marine Chlorella transformation method, in combination with the complete genome, will greatly facilitate more comprehensive mechanism studies and provide possibilities to use this species as chassis for synthetic biology to produce value-added compounds with mutual advantage in neutralization of CO 2 in commercial scales. The transgenes are integrated into the genome and can be successfully inherited for more than two years. We showed that transformants could be obtained in a dramatically time-saving manner (comparable to Saccharomyces cerevisiae) with four functional proteins expressed properly. In this study, we provided a transformation protocol for the marine Chlorella strain MEM25, which showed robust characteristics, including high production of proteins and polyunsaturated fatty acids in multiple cultivation systems over various spatial–temporal scales. However, the transformation method has not been developed in marine Chlorella spp., thus genetic engineering is hindered in exploiting the industrial potentialities of these strains. as excellent model organisms for both basic research and commercial application. The plant nature and demonstrated industrial potential facilitate Chlorella spp. The use of marine microalgae in industrial systems is attractive for converting CO 2 into value-added products using saline water and sunlight.
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